Persian Gulf Snail Crude Venom (Conus textile): A Potential Source of Anti-Cancer Therapeutic Agents for Glioblastoma through Mitochondrial-Mediated Apoptosis

Background: Research on animal toxins have shown toxicity potential cancerous cell and tissues in the cultures. Conotoxins obtained from marine cone snails show highest potential, so that several human deaths been attributed to this species of snail. These proven be valuable agents inhibit enzymes, channels proteins, nervous systems humans. Methods: We studied effects Conus textile crude venom U87MG glioma cells their mitochondria as main inducers apoptosis embryonic kidney 293 (HEK293) non-cancerous normal control cells. Cellular assessments including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay measurement caspase-3 activation well mitochondrial assays activity succinate dehydrogenase (SDH) enzyme, swelling, reactive oxygen (ROS) production, collapse membrane (MMP) cytochrome c release were performed HEK293 (as cells). Results: The results illustrated significant cytotoxic effect cells, inhibits 50% (IC50=10µg/mL) growth after 12 h exposure. Viability showed which is selectively induced induction via through signaling. also increased ROS formation, MMP decrease but not mitochondria. Conclusion; Based investigation, it concluded contains promising natural compounds fight intrinsic pathways.